WebbpRS41H, pRS41K, pRS41N, pRS42H, pRS42K, and pRS42N were obtained. They were verified by sequencing and restriction digest. CONTINUED ON NEXT PAGE. SHORT … WebbYeast plasmids pRS41N and pRS41H that confer clonNAT and hygB resistance, respectively, to N. crassa and C. parasitica. Unique restriction sites in the multiple …

酿酒酵母表达宿主菌BY4742-上海北诺生物科技有限公司

Webb1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. … Webb49 USD DHL Worldwide Shipping; Add the product to the cart to get an Online Quotation; FAQ: How to Order? FAQ: How to Pay? FAQ: What's Purchase Order(PO)? the hub downtown goldsboro nc

12 Fung al Genetics Newsletter - FGSC

WebbpRS41H containing the hphNT1 gene was adopted as the template in a polymerase chain reaction (PCR) to amplify the hphNT1 open-reading frame flanked by regions homol-ogous to URA3 DNA sequences in pSH47. The plasmid Table 1 Primers used in this study Name Sequence(5′→3′) SPT15 F TCGAGTGCTAGCAAAATGGCCGATGAGGAA CGTTTAAAGG WebbTwo plasmids that were previously used with yeast, pRS41N and pRS 41H, were found to confer clonNAT and hygromycin B resistance, respectively, in the filamentous fungi … WebbPlasmids pRS41N and pRS41H (Figure 1) were derived from pRS416 as described in Taxis and Knop (2006 ). Resistance genes in both plasmids are driven by the TEF promoter … the hub downtown dayton