Linear puromycin marker
Nettet2 µg Linear Hygromycin Marker (also sold separately as Cat. No. 631625) 2 µg Linear Puromycin Marker (also sold separately as Cat. No. 631626) 100 rxns Xfect™ transfection reagent (also sold separately as Cat. No. 631320) 50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. No. 631105) Nettet9. jan. 2024 · Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb …
Linear puromycin marker
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Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a ... Nettet27. mai 2024 · In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE-Tight-Gene X plasmids should be cotransfected with the Linear Hygromycin Marker (#631625 or #6202-1, not …
NettetLinear Selection Markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer Linear Selection Markers for … NettetPlasmid vectors using puromycin resistance as a selective marker have been developed for many methanogenic Archaea. The current vectors are constructed either with …
Nettet8. aug. 2024 · Published OnlineFirst April 30, 2013; DOI: 10.1158/0008-5472.CAN-12-3801 Cancer Tumor and Stem Cell Biology Research Cancer Stem–like Cell Marker CD44 Promotes Bone Metastases by Enhancing Tumorigenicity, Cell Motility, and Hyaluronan Production 1 2 1 Toru Hiraga , Susumu Ito , and Hiroaki Nakamura Abstract CD44, an … NettetThermo Fisher ptetone construct plus linear puromycin marker Ptetone Construct Plus Linear Puromycin Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
NettetcDNA was transfected into A549 cells with linear puromycin marker (Clontech). Puromycin-resistant clones were selected as Tet-Express inducible cells. NR0B1 expression induced by the transfection of Tet-Express with Xfect transfection reagent (Clontech) was confirmed by immunoblotting as described below.
NettetThermo Fisher ptetone construct plus linear puromycin marker Ptetone Construct Plus Linear Puromycin Marker, supplied by Thermo Fisher, used in various techniques. … mechanic business cards examplesNettetTet-One Inducible Expression System (Cat. No. 634301) 1 each pTetOne Vector Set (Cat. No. 634303; not sold separately) − 20 μl pTetOne Vector (500 ng/µl) − 20 μl pTetOne-Luc Control Vector (500 ng /µl) − 40 μl Linear Hygromycin Marker (50 ng/µl) (also sold separately as Cat. No. 631625) − 40 μl Linear Puromycin Marker (50 ng/µl) … peking mathematical journalNettethuman lung carcinoma cells are best suited for titering vectors carrying puromycin selection marker, whereas Hepa mouse hepatoma cells for those with blasticidin selection marker. page 2 ... and the linear range and sensitivity of the assay. Example: Standard Curve y = 11286x + 1048.7 R2 = 0.9723 0 5,000 10,000 15,000 20,000 25,000 30,000 peking motor show 2022Nettet我们提供具有潮霉素（Linear Hygromycin Marker）或嘌呤霉素（Linear Puromycin Marker）抗性的线性选择标记。这些标记是由标记基因、SV40启动子和SV40多聚腺 … peking muscatine hoursNettet631626 - Linear Puromycin Marker Überarbeitet am 2024-12-23 1-800-633-8253 (US) or 801-629-0667 (international). Italien Marco Marano CAV “Osp. Pediatrico Bambino … peking michigan cityNettetLinear Selection Markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer Linear Selection Markers for either hygromycin resistance (Linear Hygromycin Marker) or puromycin resistance (Linear Puromycin Marker). These markers are short, purified linear DNA fragments … peking mechanicsville menuNettetLinear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Line ... mechanic business card template