Hif1a antibody
WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. WebUnder normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O 2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia …
Hif1a antibody
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WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of …
WebAlternative Name: HIF1; HIF-1A; HIF1A; HIF-1-alpha; HIF1-alpha; BHLHE78; MOP1; PASD8 Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ ... Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. WebHIF1a/HIF-1 alpha antibody used for IHC-Fr on frozen liver sections of pair- or ethanol-fed C57BL/6J wild-type mice (Figure 1B) and hepatocyte-specific HIF1a-deficint mice /delta HepHIF1a-/- on C57BL/6 background …
WebHIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an … Web13 de mai. de 2024 · DNA was immunoprecipitated from the sonicated cell lysates using anti-HIF1A antibody (Abcam) and immunoprecipitates were recovered by addition of Salmon Sperm DNA/Protein A Agarose-50% Slurry.
WebUnder hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular …
Web21 de mar. de 2024 · HIF1A (Hypoxia Inducible Factor 1 Subunit Alpha) is a Protein Coding gene. Diseases associated with HIF1A include Retinal Ischemia and Enchondromatosis, … hill stations to visit in may monthWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-HIF-1 alpha antibody [EPR16897] (ab179483) Research with confidence – consistent and … smart bud cartridge realWebImmunocytochemistry analysis of HIF1A in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA. Samples were incubated in HIF1A monoclonal … hill stations wallpapersWeb11 de nov. de 2015 · Abstract. Background: Hypoxia Inducible Factor (HIF) regulates a cascade of transcriptional events in response to decreased oxygenation, acting from the cellular to the physiological level. This response is evolutionarily conserved, allowing the use of zebrafish (Danio rerio) as a model for studying the hypoxic response. smart bud cartridge not workingWebHIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。 Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使 … hill stead farmWebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α (D5F3M) Mouse Monoclonal Antibody (CST #79233) is ready to ship. smart buckle shoelacesWebMA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with … smart buddy nets cashless