Granuphilin c2a

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August undefined, 2024

WebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … WebJan 1, 2024 · The C2A domain of Slp-4 is known to drive this protein's ability to bind cellular PMs primarily via interaction with PIP 2 (12, 13).However, the protein retains an affinity for membranes containing the anionic lipids PS and/or PI, even in the absence of PIP 2 ().Here, we set out to discern the contributions of PIP 2 and background anionic lipid binding to …

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Webusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding afﬁnity of this protein. This suggests that granuphilin may serve as a coincidence de- WebMay 31, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before … holiday let chester uk

Multivalent lipid targeting by the calcium-independent …

NMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. WebJan 1, 2024 · The results of an in vitro binding study indicated that the C2A domain of Slp3 and Slp5 functions as an atypical Ca 2+-dependent phospholipid-binding domain ... Slp4/granuphilin was originally identified as a rabphilin-like protein associated with insulin granules in pancreatic β cells (Wang et al. 1999). WebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures … hula hoop creator

Multivalent lipid targeting by the calcium-independent …

WebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started! WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2 MLA Chanjuan Wan, et al. "Insights Into the Molecular Recognition of the Granuphilin C2a … hula hoop crazeWebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2. 7: 24184645: 2014: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. 5: 27032672: 2016: Granuphilin exclusively mediates functional granule docking to the plasma membrane. 4: holiday let cleaners isle of wight

"Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its " - Granuphilin c2a

Granuphilin c2a

Multivalent lipid targeting by the calcium-independent …

WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. Slp-4 binds vesicular Rab proteins via an N-terminal … WebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A …

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WebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin …

WebDec 12, 2013 · Proteins containing C2 domains are the sensors for Ca 2+ and PI(4,5)P 2 in a myriad of secretory pathways. Here, the use of a free-mounting system has enabled us to capture an intermediate state of Ca 2+ binding to the C2A domain of rabphilin 3A that suggests a different mechanism of ion interaction.Structural comparisons between C2A … WebLearn about Equinix DC2 carrier-neutral data center, located at 21715 Filigree Court, Ashburn, VA. See our interconnection options, certifications and more.

WebMar 7, 2002 · Granuphilin/Slp-4 is a member of the synaptotagmin-like protein family expressed in pancreatic β-cells and in the pituitary gland. We show by confocal microscopy that both granuphilin-a and -b colocalize with insulin-containing secretory granules positioned at the periphery of pancreatic β-cells. Overexpression of granuphilins in … WebJan 13, 2015 · Another example is the study of the interaction of granuphilin-C2A domain with phosphatidylinositol 4, 5-bisphosphate [PI(4, 5)P 2 ] head group and PI(4, 5)P 2 …

WebJan 27, 2015 · Granuphilin C2A has similar affinity for liposomes containing either 24% PS or 2% PIP2, but affinity increases ∼100-fold in the presence of both target lipids. Binding site(s) for the two lipids ...

WebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, … hula hoop dancer for hireWebAll Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their … hula hoop crisp boxWebAug 24, 2024 · The C2A domain of granuphilin binds strongly to plasma membranes containing PIP2 via a conserved lysine cluster (red circle) embedded in a large electropositive surface (blue oval) containing many basic residues (blue sticks). A single exposed hydrophobic residue (gray sticks) also contributes to membrane affinity. hula hoop craftWebSep 1, 2014 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P 2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. hula hoop diy wreathWebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … holiday let cleaners horncastleWeband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the … holiday let by the seaWebMay 29, 2024 · Multivalent lipid targeting by the calcium-independent C2A domain of Slp-4/granuphilin Aml A Alnaas 1, Abena Watson-Siriboe 1, Sherleen Tran , Mikias Negussie, Jack A. Henderson , J. Ryan Osterberg 1, Nara Lee Chon , Julianna Oviedo,1 Tatyana Lyakhova1, Cole Michel2, Nichole Reisdorph 2, Richard Reisdorph , Colin T. Shearn3, … holiday let cleaning cornwall