Cspg4 cre

Author: pgld

August undefined, 2024

WebCspg4Cre -Driven, LacZ-Labeled Cells Are Present in the Female Reproductive Tract Epithelium (A) X-gal stained whole-mount images of Cspg4 Cre ;R26-lacZ fl/+ mouse MD (E14) (a), neonatal oviducts and … WebA single-cell suspension was prepared by pooling the digested SSV and both adjacent PLVs from the hindlegs of Cspg4-Cre;tdT mice (n = 3), which were sequentially sorted by fluorescence activated ...

CRE Property Portfolio CP Group

WebMay 23, 2024 · Cspg4-Cre lines were genotyped by qPCR according to The Jackson Laboratory protocol. Mouse Procedures. For induction of Cre-mediated recombination in NG2-Cre ERTM mice, 1 mg of tamoxifen (Sigma-Aldrich) was injected twice daily for 5 consecutive days as previously described (Kunisaki et al., 2013. Kunisaki Y. evan gets his wings daily motion

CSPG4 Gene - GeneCards CSPG4 Protein CSPG4 Antibody

WebApr 14, 2024 · Tumor growth was impaired when Dnmt1 was disabled in ECs, but not in fibroblasts using Postn-cre mice, with orthotopically engrafted EO771 and PyMT mammary tumor cells (Fig. 1a, Supplementary Fig ... WebMar 28, 2024 · Mutation details: The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp … Web[2], CSPG4-dsRed [6 ], CSPG4-TK [2 ], CSPG4-Cre [7 ], and CSPG4-CreERT2 [8], have been generated to target peri-cytes. None of these markers, however, are pericyte-specic. For example, PDGFRβ, CD13, and RGS5 also mark vascular smooth muscle cells (vSMCs) and broblasts in addition to pericytes, while CSPG4 labels pericytes, vSMCs, and evanger\\u0027s organic cat food

Retina-specific targeting of pericytes reveals structural ... - PubMed

029926 - NG2-Cre on B6 Strain Details - The Jackson …

Web007576), Cspg4-DsRed (Tg(Cspg4-DsRed.T1)1Akik, stock number 008241, also called NG2-DsRed or NG2DsRedBAC)22 and constitutive Cspg4-Cre (B6; FVB-Tg(Cspg4-cre)1Akik/J, stock number 008533)22 were purchased from Jackson Laboratory. Pdgfrb-Cre23 mouse strain was a kind gift from Dr. Ralf Adams. Mice were genotyped by PCR. … WebMar 7, 2024 · Results: Fluorescent imaging of Cspg4-Cre;tdTomato vessels demonstrated dim PLVs and strong reporter gene expression in the adjacent superficial saphenous … evang. gymnasium und wshWebChondroitin sulfate proteoglycan 4 (CSPG4) belongs to the chondroitin sulfate group of glycosaminoglycans (GAGs) and is a type 1 transmembrane protein that harbors … evang-feldkirch.at

"WebJun 10, 2016 · Oligodendrocyte precursor cells coexpressed Pdgfra Cspg4(Fig. 2B figs.S1B 10%coexpressed cell cycle genes (fig. S2, celldivision turn- over 19days juvenilecortex Severalgenes Tmem100)identified OPCswere previously associated astro-cytes radialglia (figs.S1B, S3, S10),consistent OPCsfrom radial glia–like cells, gen-erate astrocytes ... " - Cspg4 cre

Cspg4 cre

Pericyte loss leads to circulatory failure and

WebThe transgene consists of a tamoxifen-inducible cre recombinase driven by the 2.3-kb mouse Col1a1, collagen, type I, alpha 1, promoter. The transgene insert contains a fusion product involving cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand ... WebApr 15, 2024 · The specificity and pattern of cre activation varied significantly across the three lines, under either the PDGFRβ or NG2 promoter (Pdgfrβ-CreRha, Pdgfrβ-CreCsln, …

Did you know?

WebThe specificity and pattern of cre activation varied significantly across the three lines, under either the PDGFRβ or NG2 promoter (Pdgfrβ-CreRha, Pdgfrβ-CreCsln, Cspg4-Cre). In particular, a mouse line with Cre under the NG2 promoter resulted in sparse TdTomato labeling of mural cells, allowing for an unambiguous characterization of ... WebOct 10, 2024 · Finally, the Cspg4-Cre/Esr1*:Dsp F/F mice did not show evidence of cardiac dysfunction, which would be expected on deletion of Dsp in cardiac myocytes, as reported previously. 19 Consequently, the data indicate that cardiac arrhythmias and conduction defects in the Cspg4-Cre/Esr1*:Dsp F/F mice are independent of cardiac myocytes and …

WebPlease remember that APCU will never request your personal information, online banking credentials or one time passcode via phone, email or text. If you receive a suspicious … WebMar 28, 2024 · Mutation details: A targeting vector was designed so that it contained a homology arm-flanked nlsCre cDNA (nls;nuclear localization signal), a pgk-neo cassette 3' of the nlscre, and an MC1-driven diphtheria toxin selection gene. After homologous recombination in RENKA ES cells, targeted clones had the cre cDNA inserted …

WebJan 10, 2024 · Overexpression of the chondroitin sulfate proteoglycan 4 (CSPG4) has been associated with the pathology of multiple types of such as melanoma, breast cancer, squamous cell carcinoma, mesothelioma, neuroblastoma, adult and pediatric sarcomas, and some hematological cancers. CSPG4 has been reported to exhibit a role in the growth … WebIndeed, when we tested Cspg4-Cre ( Zhu et al. 2008) and PDGFRβ-Cre ( Foo et al. 2006) BAC transgenic mice by crossing with the R26-Tomato reporter line, we found abundant labeling outside the ...

Web008533 B6;FVB- Ifi208 Tg(Cspg4-cre)1Akik /J These transgenic mice express the Cre recombinase under the control of the mouse chondroitin sulfate proteoglycan 4 ( Cspg4 ) …

WebSep 17, 2024 · Tamoxifen (TAM) inducible Cre recombinase system is an essential tool to study gene function when early ablation or overexpression can cause developmental defects or embryonic lethality. However ... evanger\u0027s recallWebAug 19, 2024 · The specificity and pattern of cre activation varied significantly across the three lines, under either the PDGFRβ or NG2 promoter (Pdgfrβ-CreRha, Pdgfrβ-CreCsln, and Cspg4-Cre). In particular, a mouse line with Cre under the NG2 promoter resulted in sparse TdTomato labeling of mural cells, allowing for an unambiguous characterization of ... evan gibbs troutmanWebJun 6, 2016 · Sec. 19144. Sec. 19144. Subject to Sections 21223 and 21224, a person who has retired from state civil service may be employed temporarily in a civil service position … evanger\u0027s super premium dry cat foodWebSep 3, 2024 · Constitutive CRE expression, which tests Wnt requirements starting in embryos, had little deleterious effect in Cspg4 Cre;Wls Fl/Fl or Pdgfra Cre;Porcn Fl … first choice haircutters niagara falls onWebThe latency of the rotarod test was markedly decreased in Cspg4‐Cre; senp1 f/f mice after cerebral ischemia (Figure 3F, Figure S4, Table S1). There was no significant difference in the Y‐maze test between Cspg4‐Cre; senp1 f/f mice and senp1 f/f mice, indicated no influence on learning and memory ability (Figure 3G,H, Figure S4, Table S1 ... first choice haircutters oakvilleWebJun 24, 2024 · The Pdgfrb-Flp; Cspg4-FSF-CreER line was characterized further by crossing to Ai14 Cre reporter mice that harbor a loxP-flanked STOP cassette that … evan gibson insuranceWebApr 6, 2024 · To address this issue, an innovative dual-promoter approach (PDGFRβ-Flp:CSPG4-FSF-CreER), in which Cre expression is driven by both PDGFRβ and … first choice haircutters orillia hours