Cisplatin dna cross-linking

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August undefined, 2024

WebCisplatin Medicine & Life Sciences 74%. DNA Repair Medicine & Life Sciences 50%. DNA Medicine & Life Sciences 46%. DNA Adducts Medicine & Life Sciences 34%. ... (II) and Modification of Platinum-induced DNA Interstrand Cross-Linking. / Swinnen, Lode J.; Barnes, Diane M.; Fisher, Susan G. et al. WebDNA interstrand crosslinks (ICLs) formed by antitumor agents, such as cisplatin or mitomycin C, are highly cytotoxic DNA lesions. Their repair is believed to be triggered primarily by the stalling of replication forks at ICLs in S-phase. There is, however, increasing evidence that ICL repair can also occur independently of replication.

Interstrand Cross‐Links in Cisplatin‐ or …

WebThe drug reacts with nucleophilic sites in DNA forming monoadducts as well as intra- and interstrand crosslinks. DNA-cisplatin adducts are specifically recognized by several … phixton wt-04

(PDF) Enhanced repair of DNA interstrand crosslinking in ovarian …

WebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to … WebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis … WebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 … phix texas

A structure-based mechanism of cisplatin resistance mediated by ... - PNAS

WebRecent studies have shown that the majority of proteins cross-linked to DNA by cisplatin in situ are nuclear matrix proteins ( 2 - 4 ). We have also shown that cisplatin cross-links … WebJul 23, 1996 · Abstract cis-Diamminedichloroplatinum (II) (cisplatin) is a widely used anticancer drug that binds to and crosslinks DNA. The major DNA adduct of the drug results from coordination of two adjacent guanine bases to platinum to form the intrastrand crosslink cis- [Pt (NH3)2 [d (GpG)-N7 (1), -N7 (2)]] (cis-Pt-GG). phixton torchWebJun 24, 2011 · For example, cisplatin, the most widely used crosslinking drug, causes damage that is estimated to comprise of 90% intrastrand crosslinks (predominantly crosslinks between adjacent purine... phixton wt-04 flashlight

"WebCisplatin is a platinum-based drug which acts by binding to DNA by forming intrastrand cross-link conjugate which eventually leads to apoptosis due to the activation of p53, … " - Cisplatin dna cross-linking

Cisplatin dna cross-linking

Isolation of Proteins Cross-linked to DNA by Cisplatin

WebTumour cells isolated from 50 ovarian cancer patients were treated ex vivo with 100 μ M cisplatin for 1 h and crosslink formation and repair (unhooking) measured. ... in cellular mechanisms such as drug transport or detoxification. In contrast, the percentage repair (unhooking) of DNA interstrand crosslinks was much greater in the group of ... WebCisplatin is one of the most widely used agents in cancer chemotherapy. Its mode of action is cross-linking of the DNA, which can kill cells. But which part of the genome is more …

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WebJul 8, 2009 · The two opposing guanine residues involved in the cross-link were identified via limited alkaline hydrolysis. The kinetics of aquated cisplatin binding to the BBD RNA, a related RNA hairpin, and its DNA hairpin analogue were investigated in an ionic background of 0.1 M NaNO (3) and 1 mM Mg (NO (3)) (2). WebDec 22, 2024 · Cisplatin has been widely used for the treatment of cancer and its antitumour activity is attributed to its capacity to form DNA adducts, predominantly at guanine residues, which impede cellular processes …

WebFeb 21, 2000 · Most simple cis-PtA2G2 complexes that model the G-G cross-link DNA lesions caused by the clinically used anticancer drug cis-PtCl2 (NH3)2 undergo large fluxional motions at a rapid rate (A2 = two amines or a diamine; G = guanine derivative). Webwww.ncbi.nlm.nih.gov

WebThe DNA binding is similar to cisplatin, and the complex forms a highly stereoselective adduct on DNA, but not for reactions with mono- and dinucleotides. 98 In cell lines with previously determined mechanisms of cell resistance, AMD473 showed little cross-resistance compared with cisplatin or carboplatin. WebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such …

WebROS can induce single or double-strand breakage, nucleotide alterations, deoxyribose changes, and DNA cross-linking in DNA. If oxidative DNA damage is not repaired before DNA replication, it could lead to cell death, DNA mutation, replication mistakes, and genomic instability (Cooke et al., 2003; Schupp et al., 2016).

WebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis when repair proves impossible. phixton customer serviceWebOct 28, 2003 · A dominating preference for initial attack at the N7-position of guanine is established experimentally, which is a crucial first step for the formation of a 1,2-intrastrand cross-link of adjacent guanine bases that leads to bending and unwinding of DNA. phix toner targetWebCisplatin induces DNA damage through inter-strand or intra-strand cross-linking of DNA . Its dose-limiting toxicity is nephrotoxicity, peripheral nerve toxicity, and toxicity to the cochlea (the inner ear toxicity) . Paclitaxel is a microtubule poison that … phix the rapperWebCisplatin produces DNA cross-linking and is used to treat medulloblastoma, head and neck, ovarian, germ cell, cervical, lung and bladder cancers. Cisplatin produces a … phix timeWebThis chapter deals with the interstrand cross-links in cisplatin- or transplatin-modified DNA and focuses on three main aspects: the formation of the interstrand cross-links, the distortions induced in the DNA double helix and the potential use of the interstrand cross-linking reaction in the context of antisense and antigene strategies. phixton tactical led flashlight high lumensWebFeb 28, 2024 · Indeed, while cisplatin probably undergoes an efflux effect, compound 19 shows a time-dependent accumulation in cells and a greater ability to cross the membrane. DNA interaction has been confirmed for both complexes due to the presence of the triphenylphosphine hydrophobic moiety and the trans geometry of bromide groups. tssm narhe logoWebJun 20, 2024 · It has long been established that the mechanism of cis- DDP involves DNA intrastrand cross-link formation ( 6, 7 ), altering the molecular conformation of the double helix leading to apoptosis. phix tobacco cartridge doesnt work